浓度: 20,000 U/ml
|Exonuclease I (E. coli) (20,000 U/ml)||RM20519|
|10X Exonuclease I Reaction Buffer||RM20130|
|Product Source||An E.coli strain that carries the cloned Exo I gene from E. coli NM554.|
|Unit Definition||One unit is defined as the amount of enzyme that will catalyze the release of 10 nmol of acid-soluble nucleotide in a total reaction volume of 50 μl in 30 minutes at 37°C in 1X Exonuclease I Reaction Buffer with 0.17 mg/ml single-stranded [3H]-DNA.|
|Storage Conditions||10 mM Tris-HCl, 100 mM NaCl, 5 mM β-ME, 0.5 mM EDTA, 100 μg/ml BSA, 50% Glycerol, pH 7.5 @ 25°C|
|Reaction Conditions||1X Exonuclease I Reaction Buffer, Incubate at 37°C|
|Reaction Buffer or Mix||1X Exonuclease I Reaction Buffer: |
67 mM Glycine-KOH, 6.7 mM MgCl2, 10 mM β-ME, pH 9.5 @ 25°C
|Heat Inactivation||80°C for 20 min|
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