产品和服务 / 产品类型 / 细胞系&裂解液 / KO细胞裂解液

EZH2 Knockout HeLa Cell Lysate, Homozygous (RM02009)

说明书收藏

Genome sequence analysis of PCR products from parental (WT) and EZH2 knockout (KO) HeLa cells, using sanger sequencing.

Western blot analysis of extracts from parental (Control) and EZH2 knockout (KO) HeLa cells, using EZH2 antibody (A11085) at 1:1000 dilution.

货号: RM02009

详细信息

靶点

EZH2

细胞系

HeLa

突变描述

EZH2 Knockout HeLa Cell Line is engineered from HeLa cell line with Gene-Editing technology.
Allele-1:1bp insertion in exon3
Allele-2:13bp deletion in exon3

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.

敲除验证

Sanger Sequencing

产品组成

1 vial parental cell Lysate and 1 vial knockout cell Lysate

裂解液量

50μL, 2μg/μL.

使用方法

To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.

物种

Human

保存条件

Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.

运输条件

4℃

背景信息

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Feb 2011]

基因ID

Swiss Prot

别名

ENX-1; ENX1; EZH1; EZH2b; KMT6; KMT6A; WVS; WVS2

研究领域

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