TJP2 Knockout HeLa Cell Lysate, Homozygous (RM02051)

说明书收藏

Genome sequence analysis of PCR products from parental (WT) and TJP2 knockout (KO) HeLa cells, using sanger sequencing.

All(1)|

货号: RM02051

详细信息

靶点

TJP2

细胞系

HeLa

突变描述

TJP2 Knockout HeLa Cell Line is engineered from HeLa cell line with Gene-Editing technology.
Allele-1:67bp deletion in exon1
Allele-2:67bp deletion in exon1

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.

敲除验证

Sanger Sequencing

产品组成

1 vial parental cell Lysate and 1 vial knockout cell Lysate

裂解液量

50μL, 2μg/μL.

使用方法

To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.

物种

Human

保存条件

Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.

运输条件

4℃

背景信息

This gene encodes a zonula occluden that is a member of the membrane-associated guanylate kinase homolog family. The encoded protein functions as a component of the tight junction barrier in epithelial and endothelial cells and is necessary for proper assembly of tight junctions. Mutations in this gene have been identified in patients with hypercholanemia, and genomic duplication of a 270 kb region including this gene causes autosomal dominant deafness-51. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

基因ID

9414

Swiss Prot

Q9UDY2

别名

C9DUPq21.11; DFNA51; DUP9q21.11; PFIC4; X104; ZO2

研究领域