TBXT Knockout HeLa Cell Lysate, Homozygous (RM02549)

说明书收藏

Genome sequence analysis of PCR products from parental (WT) and TBXT knockout (KO) HeLa cells, using sanger sequencing.

All(1)|

货号: RM02549

详细信息

靶点

TBXT

细胞系

HeLa

突变描述

TBXT Knockout HeLa Cell Line is engineered from HeLa cell line with Gene-Editing technology.
Allele-1:85bp deletion in exon2;Allele-2:85bp deletion in exon2

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.

敲除验证

Sanger Sequencing

产品组成

1 vial parental cell Lysate and 1 vial knockout cell Lysate

裂解液量

50μL, 2μg/μL.

使用方法

To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.

物种

Human

保存条件

Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.

运输条件

4℃

背景信息

The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]

基因ID

6862

Swiss Prot

O15178

别名

SAVA; TFT

研究领域