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This standard curve is only for demonstration purposes. A standard curve should be generated for eachassay.
No significant cross-reactivity or interference was observed.
Placed at 37°C for 3 days, the stability of the standard curve all conformto CV <10%.
* This package insert must be read in its entirety before using this product.
Human CD14 Sanwich ELISA Immunoassay
| Recommended Concentration | Sample | |
|---|---|---|
| ELISA Capture | 0.25-1ug/mL | Rabbit anti-Human Cluster Of Differentiation 14 mAb (CAP) (CAP)(Cat. No.RM17908) |
| ELISA Detection | 0.0025-0.01ug/mL | Rabbit anti-Human Cluster Of Differentiation 14 mAb (DET)(Cat. No.RM17909) |
| Standard | 15.63-1000pg/mL | Recombinant Human CD14 Protein |
Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655).In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:23264655, PubMed:22265692).Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135).Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211).Binds electronegative LDL (LDL) and mediates the cytokine release induced by LDL (PubMed:23880187)
