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Rabbit anti-Human IgM mAb(DET) (RMK0104)

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

No significant cross-reactivity or interference was observed.

Placed at 37°C for 3 days, the stability of the standard curve all conform to CV <10%.

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货号: RMK0104
促销价:   ¥2100
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详细信息

产品名称
Rabbit anti-Human IgM mAb(DET)
货号
RMK0104
免疫原
Native Human IgM
纯化方式
Affinity purification
同种型
Rabbit IgG
存储溶液
Supplied as a 0.2um filtered solution in PBS with 0.05%ProClin 300,PH 7.4.
CrossReactivity
No cross-reactivity in ELISA assay withrecombinant
M.IgM,R.IgM,M.IgE,R.IgA
存储条件
Store at -20℃. Avoid freeze / thaw cycles. Preservative:0.05%ProClin 300.Avoid repeated freeze-thaw cycles.

* This package insert must be read in its entirety before using this product.

应用

Human IgM Sandwich ELISA Immunoassay

 Recommended
Concentration
Sample
ELISA Capture1-4ug/mLRabbit anti-Human IgM mAb(CAP)(Cat. No.RMK0103)
ELISA Detection0.0165-0.066ug/mLRabbit anti-Human IgM mAb(DET)(Cat. No.RMK0104)
Standard0.31-20ng/mLRecombinant Human IgM Protein

背景信息

Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).

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