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活性验证

Recombinant Human SLC3A2/CD98 Protein (RP00014)

文献引用 (1) 说明书 收藏

Recombinant Human SLC3A2/CD98 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 45 kDa.

Immobilized Recombinant Human SLC3A2/CD98 Protein at 5 μg/mL (100 μL/well) can bind ITGB1 with a linear range of 0.0195-1.216 μg/mL.

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货号: RP00014
促销价：   ￥1000
货    期：现货产品
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详细信息

种属
Human
表达宿主
Baculovirus-Infected Sf9 Cells
Calculated MW
46.85 kDa
Observed MW
45 kDa
标签
No tag
纯度
> 97% by SDS-PAGE.
内毒素
< 1.0 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述
Recombinant Human SLC3A2/CD98 Protein is produced by insect cell-baculovirus expression system. The target protein is expressed with sequence (Arg206-Ala630) of human SLC3A2 (Accession #NP_002385.3).
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
Measured by its binding ability in a functional ELISA.Immobilized Human SLC3A2 Protein at 5 μg/mL (100 μL/well) can bind ITGB1 with a linear range of 0.0195-1.216 μg/mL.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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文献引用 (1)

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Publishing research using RP00014? Please let us know so that we can cite the reference in this datasheet.

背景信息

This protein is a member of the solute carrier family and encodes a cell surface, transmembrane protein. The protein exists as the heavy chain of a heterodimer, covalently bound through di-sulfide bonds to one of several possible light chains. The encoded transporter plays a role in regulation of intracellular calcium levels and transports L-type amino acids. Alternatively spliced transcript variants, encoding different isoforms, have been characterized.

基因ID
6520
Swiss Prot
P08195
别名
4F2; 4F2HC; 4T2HC; CD98; CD98HC; MDU1; NACAE;SLC3A2;4F2HC;4T2HC;CD98;CD98HC;MDU1;NACAE
研究领域
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