Recombinant Human CSF1R/M-CSF R/CD115 Protein (RP00268)

Recombinant Human CSF1R/M-CSF R/CD115 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 70-100 kDa.

Immobilized Recombinant Human M-CSF at 2μg/mL (100 μL/well) can bind Recombinant Human CSF1R with a linear range of 50-200 ng/mL.

Recombinant Human CSF1R inhibits the GCSF-induced proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this ettect is typically 0.095-0.38 ng/mL.

Immobilized Human CSF-1/M-CSF(Cat. RP01221) at 2μg/mL (100 μL/well) can bind Human CSF1R with a linear range of 2-185 ng/mL.

All(4)|

货号: RP00268

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

55.34 kDa

Observed MW

70-100 kDa

标签

C-His

纯度

> 95% by SDS-PAGE.

内毒素

< 0.1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.

描述

Recombinant Human CSF1R/M-CSF R/CD115 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ile20-Glu512) of human CSF1R (Accession #NP_005202.2) fused with a 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human M-CSF at 2 μg/mL (100 μL/well) can bind Recombinant Human CSF1R with a linear range of 50-200 ng/mL.
2.Measured by its ability to inhibit the GCSF-induced proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this ettect is typically 0.095-0.38 ng/mL.
3.Measured by its binding ability in a functional ELISA.Immobilized Human CSF-1/M-CSF(Cat. RP01221) at 2μg/mL (100 μL/well) can bind Human CSF1R with a linear range of 2-185 ng/mL.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

The recombinant human CSF1R/GST chimera consists of 667 amino acids with a molecular weight of 76 KDa. It migrates as an about 75 KDa band in SDS-PAGE under reducing conditions.M-CSFR encoded by the proto-oncogene c-fms is the receptor for colony stimulating factor 1 (CSF1R), a cytokine involved in the proliferation, differentiation, and activation of macrophages.This cell surface glycoprotein is consisted by an extracellular ligand-binding domain, a single membrane-spanning segment, and an intracellular tyrosine kinase domain. CSF1R is a receptor for a cytokine called colony stimulating factor 1, The protein encoded by the CSFR1 gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most, if not all, of the biological effects of this cytokine. Ligand binding activates CSFR1 through a process of oligomerization and transphosphorylation . Mutations in CSF1R are associated with chronic myelomonocytic leukemia and type M4 acute myeloblastic leukemia. Increased levels of CSF1R1 are found in microglia in Alzheimer's disease and after brain injuries. The role of CSF1 and CSF1R in normal and neoplastic mammary development that may elucidate potential relationships of growth factor-induced biological changes in the breast during pregnancy and tumor progression.

基因ID

1436

Swiss Prot

P07333

别名

CSF1R;C-FMS;CD115;CSF-1R;CSFR;FIM2;FMS;HDLS;M-CSF-R;BANDDOS

研究领域