产品中心 / 产品类型 / 重组蛋白 / 生物标志物 & CD分子

Recombinant Human IL-6RA/CD126 Protein (RP00269)

说明书 联系我们 收藏

Recombinant Human IL-6RA/CD126 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 60-70 kDa.

The purity of Human IL-6 R alpha Protein (Cat.RP00269) was greater than 90% as determined by SEC-HPLC.

Immobilized Human IL6R at 1 μg/mL (100 μL/well) can bind Human IL-6 with a linear range of 2-15ng/mL.

All(3)|
货号: RP00269
促销价：   ￥1000
货    期：现货产品
抗体定制服务咨询

文献引用 (0)

详细信息

种属
Human
表达宿主
HEK293 cells
描述
Recombinant Human IL-6RA/CD126 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu20-Asp358) of human IL-6 R alpha (Accession #NP_000556.1) fused with a 6×His tag at the C-terminus.
标签
C-His
纯度
> 92% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
生物活性
Measured by its binding ability in a functional ELISA. Immobilized Human IL6R at 1 μg/mL (100 μL/well) can bind Human IL-6 with a linear range of 2-15 ng/mL.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
储存
Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
=
÷

背景信息

The soluble form of recombinant human IL6R consists of 357 amino acids with a molecular weight of 40 kDa. It migrates with an apparent molecular mass of 60-65 kDa due to glycosylation in SDS-PAGE under reducing conditions. Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a potent pleiotropic cytokine that regulates cell growth and differentiation of various tissues, and is known particularly for its role in the immune response and acute phase reactions. The low concentration of a soluble form of IL-6 receptor (sIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a sIL-6R protein are able to mediate IL-6 signals into the cells through the interaction of gp13. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp13 and inducing intracellular signalling. Through this so-called 'trans-signalling' mechanism, IL-6 is able to stimulate cells that lack an endogenous mIL-6R. Dysregulated production of IL6 and IL6R are implicated in the pathogenesis of several inflammatory diseases and malignancies, and it has been reported that a humanized anti-IL6R monoclonal antibody is a promising agent applicable to the therapeutic approach for IL6 driven diseases.

基因ID
3570
Swiss Prot
P08887
别名
IL6R;CD126;IL-6R-1;IL-6RA;IL6Q;IL6RA;IL6RQ;gp80
研究领域
>