Recombinant Human B7-H1/PD-L1/CD274 Protein (RP00331)

说明书收藏

Human PD-L1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Human PD-L1 is greater than 95% as determined by SEC-HPLC.

Immobilized Human PD-1, mFc Tag at 1 μg/mL (100 μL/well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC₅₀ of 0.17 μg/mL determined by ELISA (QC Test).

Human PD-L1, hFc Tag captured on CM5 Chip via Protein A can bind Human PD-1, His Tag with an affinity constant of 2.20 μM as determined in SPR assay (Biacore T200).

All(4)|

货号: RP00331

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

52 kDa

Observed MW

68-80 kDa

标签

C-hFc

纯度

> 95% by SDS-PAGE；> 95% by HPLC

内毒素

< 1 EU/μg of the protein by LAL method

制剂

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

描述

Recombinant Human PD-L1/B7-H1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Phe19-Arg238) of Human PD-L1/B7-H1 (Accession #Q9NZQ7-1) fused with a C-hFc tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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背景信息

B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.

基因ID

29126

Swiss Prot

Q9NZQ7-1

别名

CD274; PDL1; PD-L1; PD-L1B7 homolog 1;B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1

研究领域