Recombinant Human 5'-Nucleotidase/NT5E/CD73 Protein (RP00410)

说明书收藏

Human CD73 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Human CD73 is greater than 95% as determined by SEC-HPLC.

Immobilized Human CD73, hFc Tag at 2 μg/mL (100 μL/well) on the plate. Dose response curve for Biotinylated Anti-CD73 Antibody, hFc Tag with the EC₅₀ of 22.6ng/mL determined by ELISA (QC Test).

All(3)|

货号: RP00410

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

84.5 kDa

Observed MW

85-100 kDa

标签

C-hFc

纯度

> 95% by SDS-PAGE；> 95% by HPLC

内毒素

< 1 EU/μg of the protein by LAL method

制剂

Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 120 mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.

描述

Recombinant Human 5'-Nucleotidase/NT5E/CD73 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Trp27-Lys547) of Human 5'-Nucleotidase/NT5E/CD73 (Accession #P21589-1) fused with a C-hFc tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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背景信息

CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A? deficiency of CD73 occurs in a variety of immunodeficiency diseases.

基因ID

4907

Swiss Prot

P21589-1

别名

CD73; NT5E; NTE; 5' nucleotidase (CD73); 5'-NT; 5-NT; E5NT; EC 3.1.3.5; eN; eNT; NT; NT55'-nucleotidase; NT5; NTE; CALJA

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