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Recombinant Human IL-15RA/CD215 Protein (RP00437)

Human IL-15RA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Human IL-15RA is greater than 95% as determined by SEC-HPLC.

Immobilized Human IL-15, No Tag at 0.5 μg/mL (100 μL/Well) on the plate. Dose response curve for Human IL-15RA, hFc Tag with the EC50 of 30.2ng/mL determined by ELISA.

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货号: RP00437
促销价:   ¥3200
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详细信息

种属
Human
表达宿主
HEK293 cells
Calculated MW
45.1 kDa
Observed MW
70-80 kDa
标签
C-hFc
纯度
> 95% by SDS-PAGE;> 95% by HPLC
内毒素
< 1 EU/μg of the protein by LAL method
制剂
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
描述
Recombinant Human IL-15RA/CD215 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ile31-Thr205) of Human IL-15RA/IL-15 R alpha/CD215 (Accession #Q13261-1) fused with a C-hFc tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度,快速计算您需要添加溶液的体积吧!
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背景信息

Interleukin-15 receptor alpha (IL-15R alpha) is a high affinity IL-15 binding protein that is crucial for mediating IL-15 functions such as memory CD8 T cell proliferation and NK, NK/T cell, and intestinal intraepithelial lymphocyte development.

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