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FITC-Labeled Recombinant Human HER1/ERBB1/EGFR (25-378) Protein (RP00500FLQ)

FITC-Labeled Human EGFRVIII on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

FACS Analysis of Anti-EGFRVIII CAR Expression. 293T cells were transfected with anti-EGFRVIII-scFv and His tag. Cells were incubated with 5 μg/mL FITC-Labeled Human EGFRVIII, His Tag and FITC-labeled protein control. Non-transfected 293T cells and FITC-labeled protein control were used as negative control.

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货号: RP00500FLQ
促销价:   ¥9600
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详细信息

种属
Human
表达宿主
HEK293 cells
Calculated MW
41.6 kDa
Observed MW
68-80 kDa
标签
C-His&Avi
纯度
> 95% by SDS-PAGE
内毒素
< 1 EU/μg of the protein by LAL method
制剂
Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
描述
FITC-Labeled Recombinant Human ERBB1/HER1/EGFR (25-378) Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu25-Ser378) of Human ERBB1/HER1/EGFR (25-378) (Accession #NP_001333870.1) fused with a C-His&Avi tag at the C-terminus.
储存
Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。

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请在蛋白复溶计算器中输入蛋白总质量和所需终浓度,快速计算您需要添加溶液的体积吧!
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背景信息

The epidermal growth factor receptor (EGFR) is overexpressed in a variety of human epithelial tumors, often as a consequence of gene amplification. Tumors with EGFR gene amplification frequently contain EGFR gene rearrangements, with the most common extracellular domain mutation being EGFRvIII. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.

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