Recombinant Human CSF2RA/GM-CSF R alpha/CD116 Protein (RP00507)

Human GM-CSF R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Human GM-CSF R alpha is greater than 95% as determined by SEC-HPLC.

Immobilized Human GM-CSF, No Tag at 5 μg/mL (100 μL/Well) on the plate. Dose response curve for Human GM-CSF R alpha, His Tag with the EC₅₀ of 1.0 μg/mL determined by ELISA.

Immobilized Human GM-CSF R alpha, His Tag at 1 μg/mL (100 μL/Well) on the plate. Dose response curve for Anti-GM-CSF R alpha Antibody, hFc Tag with the EC₅₀ of 6.6ng/mL determined by ELISA (QC Test).

Human GM-CSF R alpha , His Tag captured on CM5 Chip via anti-His antibody can bind Human GM-CSF, No Tag with an affinity constant of 0.79nM as determined in SPR assay (Biacore T200).

All(5)|

货号: RP00507

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

35.3 kDa

Observed MW

60-70 kDa

标签

C-His

纯度

> 95% by SDS-PAGE；> 95% by HPLC

内毒素

< 1 EU/μg of the protein by LAL method

制剂

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

描述

Recombinant Human CSF2RA/GM-CSF R alpha/CD116 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu23-Gly320) of Human CSF2RA/GM-CSF R alpha/CD116 (Accession #P15509-1) fused with a C-His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

客户数据及评论 (0)

折叠内容

背景信息

In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua.

基因ID

1438

Swiss Prot

P15509-1

别名

GM-CSF-R-alpha; GMCSFR-alpha; GMR-alpha; CSF2R; CSF2RY; CSF2RA; CDw116; CD116; CSF2RAX; CSF2RAY; CSF2RX; GMCSFR; GMR; SMDP4

研究领域