Biotinylated Recombinant Human CD3E&CD3D Protein (RP00627B)

说明书收藏

Biotinylated Human CD3E&CD3D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human CD3E&CD3D was greater than 95% as determined by SEC-HPLC.

Immobilized Biotinylated Human CD3E&CD3D, hFc Tag at 1 μg/mL (100 μL/Well) on streptavidin (5 μg/mL) precoated plate. Dose response curve for OKT3, mFc Tag with the EC₅₀ of 42.1ng/mL determined by ELISA.

All(3)|

货号: RP00627B

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

40 kDa (CD3E), 35.4 kDa (CD3D)

Observed MW

48-60 kDa

标签

C-hFc&Avi

纯度

> 95% by SDS-PAGE；> 95% by HPLC

内毒素

< 1 EU/μg of the protein by LAL method

制剂

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

描述

Biotinylated Recombinant Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D)) of Human CD3E&CD3D/CD3 epsilon&CD3 delta (Accession #P07766(CD3E)&P04234(CD3D)) fused with a C-hFc&Avi tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

T-cell surface glycoprotein CD3 epsilon&CD3 delta chain, also known as CD3E&CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.

基因ID

916&915

Swiss Prot

P07766(CD3E)&P04234(CD3D)

别名

CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon

研究领域