Recombinant Human IL-1R1/CD121a Protein (RP01036)

Recombinant Human IL-1R1/IL-1RT1/CD121a Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 55 kDa.

Immobilized Recombinant Human IL1RA at 5μg/mL (100 μL/well) can bind Recombinant Human IL1R1 with a linear range of 63-252 ng/mL.

Immobilized Human IL-1 beta (Catalog: RP00002) at 10 μg/mL (100 μL/well) can bind Biotinylated Human IL-1R1 (Catalog: RP01036) with a linear range of 0.002-1 μg/mL.

All(3)|

货号: RP01036

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

36.70 kDa

Observed MW

48-65 kDa

标签

C-His

纯度

> 95% by SDS-PAGE.

内毒素

< 0.1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.

描述

Recombinant Human IL-1R1/CD121a Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp21-Thr332) of human IL1R1 (Accession #NP_000868.1) fused with a 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human IL1RA at 5 μg/mL (100 μL/well) can bind Recombinant Human IL1R1 with a linear range of 63-252 ng/mL.
2.Measured by its binding ability in a functional ELISA. Immobilized Human IL-1 beta (Catalog: RP00002) at 10 μg/mL (100 μL/well) can bind Biotinylated Human IL-1R1 (Catalog: RP01036) with a linear range of 0.002-1 μg/mL.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

Interleukin 1 receptor, type I (IL-1R1) also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor. The protein encoded by this gene is a cytokine receptor that belongs to the interleukin-1 receptor family. This protein is a receptor for interleukin 1 alpha (IL1A), interleukin 1 beta (IL1B), and interleukin 1 receptor antagonist (IL1RA). The shape change and subsequent repair processes are IL-1β activity-dependent, acting through the IL-1 type 1 receptor (IL-1R1), as co-application of the IL-1type 1 receptor antagonist protein (IL-1ra) blocks IL-1β induced effects. This gene along with interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 2 (IL1RL2), and interleukin 1 receptor-like 1 (IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. It has been suggested that the Type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling Type I receptor complex. Recombinant IL-1 soluble receptor Type I is a potent antagonist of IL-1 action.

基因ID

3554

Swiss Prot

P14778

别名

IL1R1;CD121A;D2S1473;IL-1R-alpha;IL1R;IL1RA;P80

研究领域