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Active Recombinant Human FGF-2/bFGF Protein (RP01042)

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Recombinant Human FGF-2/bFGF Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 17 kDa.

Immobilized recombinant human FGF2 at 1 μg/mL (100 μL/well) can bind recombinant human FGFR4 with a linear range of 30-125 ng/mL.

Immobilized Human FGF2 at 0.5 μg/mL (100 μL/well) can bind Human GPC3 with a linear range of 7-20ng/mL.

Recombinant Human FGF-2 promotes the proliferation of Balb3T3 mouse embryonic fibroblasts cells. The ED50 for this effect is 0.05-0.21 ng/mL, corresponding to a specific activity of 4.76×106~2.00×107 units/mg.

Recombinant Human VEGFA(40 ng/mL, Cat. RP01162) and bFGF(50 ng/mL) induce mesoderm cells to differentiate into hematopoietic stem and progenitor cells. After 4 days induction, pebbly-like CD43+ hematopoietic stem and progenitor cells appeared in the hematogenic endothelium.(Customer feedback data)

Primary neural stem cells were cultured with a final concentration of 20 ng/mL FGF2, and as shown in the figure, the size of the suspended neural stem cells gradually increased.(Customer feedback data)

All(6)|
货号: RP01042
促销价：   ￥380
货    期：现货产品
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详细信息

种属
Human
表达宿主
E. coli
描述
Active Recombinant Human FGF-2/bFGF Protein is produced by E. coli expression system. The target protein is expressed with sequence (Pro143-Ser288) of human FGF2 (Accession #NP_001997.5).
标签
No tag
纯度
> 95% by SDS-PAGE.
内毒素
< 1.0 EU/μg of the protein by LAL method.
生物活性
1.Measured by its binding ability in a functional ELISA. Immobilized Human FGF2 at 0.5 μg/mL (100 μL/well) can bind Human GPC3 with a linear range of 7-20 ng/mL.
2.Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.635-2.54 ng/mL, corresponding to a specific activity of 3.94 × 105~1.57 × 106 units/mg.
3.Recombinant Human VEGFA(40 ng/mL, Cat. RP01162) and bFGF(50 ng/mL) induce mesoderm cells to differentiate into hematopoietic stem and progenitor cells. After 4 days induction, pebbly-like CD43+ hematopoietic stem and progenitor cells appeared in the hematogenic endothelium.
4.The primary neural stem cells were cultured with 20 ng/mL bFGF and observed every 24 h. Results showed that the particle size of the suspended neural stem cells gradually increased.
制剂
Lyophilized from a 0.22 μm filtered solution of 20mM Tris，150 mM NaCl,pH7.5.Contact us for customized product form or formulation.
储存
Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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文献引用 (1)

折叠内容

Publishing research using RP01042? Please let us know so that we can cite the reference in this datasheet.

背景信息

基因ID
2247
Swiss Prot
P09038-4
别名
BFGF; FGF-2; FGFB; HBGF-2;FGF2;FGF-2;FGFB;HBGF-2;Basic FGF; BFGF; fibroblast growth factor 2
研究领域
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