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Recombinant Mouse TNF-alpha Protein (RP01071)

Recombinant Mouse TNF-alpha Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 18 kDa.

The purity of Mouse TNF-alpha Protein (Cat.RP01071) was greater than 95% as determined by SEC-HPLC.

Recombinant Mouse TNF-α protein was measured by NF-κB reporter assay in HEK293 cells .The ED50 for this effect is 40-200 pg/mL.

Immobilized recombinant Mouse TNFα at 2 μg/mL (100 μL/well) can bind Human TNFRSF1A with a linear range of 4-53 ng/mL.

Immobilized Recombinant Mouse TNF-α at 2 μg/mL (100 μL/well) can bind Mouse TNFRSF1B with a linear range of 0.02-0.77 ng/mL.

Recombinant Mouse TNF-alpha induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 0.025-0.1 pg/mL.

All(6)|
货号: RP01071
促销价:   ¥1250
货    期:现货产品
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详细信息

种属
Mouse
表达宿主
HEK293 cells
描述
Recombinant Mouse TNF-alpha Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu80-Leu235) of mouse TNF-alpha (Accession #NP_038721.1) fused with a 6×His tag at the C-terminus.
标签
C-His
纯度
> 87% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
生物活性
1.Recombinant Mouse TNF-α protein was measured by NF-κB reporter assay in HEK293 cells. The ED50 for this effect is 40-200 pg/mL.
2.Measured by its binding ability in a functional ELISA. Immobilized Mouse TNF-α at 2 μg/mL (100 μL/well) can bind Mouse TNFRSF1B with a linear range of 0.02-0.77 ng/mL.
3.Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 0.025-0.1 pg/mL.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
储存
Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度,快速计算您需要添加溶液的体积吧!
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背景信息

基因ID
Swiss Prot
别名
DIF; Tnfa; TNF-a; TNFSF2; Tnlg1f; Tnfsf1a; TNFalpha; TNF-alpha;TNF
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