Recombinant Human CD6 Protein (RP01081)

说明书收藏

Recombinant Human CD6 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 110 kDa.

Immobilized recombinant Human CD166 at 1 μg/mL (100 μL/well) can bind Human CD6 with a linear range of 4-389ng/mL.

All(2)|

货号: RP01081

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

67.36 kDa

Observed MW

110-130 kDa

标签

C-hFc&His

纯度

> 92% by SDS-PAGE.

内毒素

< 0.1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.

描述

Recombinant Human CD6 Protein is produced by HEK293cells expression system. The target protein is expressed with sequence (His18-Glu398) of human CD6 (Accession #NP_006716.3) fused with an Fc, 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

1.Measured by its binding ability in a functional ELISA. Immobilized Human CD166 at 1 μg/mL (100 μL/well) can bind Human CD6 with a linear range of 4-389 ng/mL.
2.Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8×104 cells/well are added to CD6 coated plates (5 μg/mL and 100 μL/well) in the ppresence of 10μg/ml PHA, approximately 60-70% cells will adhere specifically after 60 minutes at 37℃.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。