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Recombinant Human FLT-3/FLK-2 Protein (RP01138)

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Recombinant Human FLT-3/FLK-2 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 85-95 kDa.

Immobilized recombinant Human FLT3 at 0.5 μg/mL (100 μL/well) can bind FLT3L with a linear range of 0.05-1.3 ng/mL.

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货号: RP01138
促销价：   ￥3000
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详细信息

种属
Human
表达宿主
HEK293 cells
描述
Recombinant Human FLT-3/FLK-2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Asn541) of human FLT3 (Accession #NP_004110.2) fused with a 6×His tag at the C-terminus.
标签
C-His
纯度
> 85 % by SDS-PAGE.
内毒素
< 1.0 EU/μg of the protein by LAL method.
生物活性
Measured by its binding ability in a functional ELISA.Immobilized Human FLT3L at 0.5 μg/mL (100 μL/well) can bind FLT3 with a linear range of 0.05-1.3 ng/mL.
制剂
Lyophilized from a 0.22 μm filtered solution PBS, pH 7.4.Contact us for customized product form or formulation.
储存
Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

This protein is a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.

基因ID
2322
Swiss Prot
P36888
别名
FLT3;CD135;FLK-2;FLK2;STK1
研究领域
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