Recombinant Human FGF-1/aFGF Protein (RP01242)

Recombinant Human FGF-1/aFGF Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 20 kDa.

The purity of human FGF acidic/FGF1 Protein (Cat.RP01242) was greater than 95% as determined by SEC-HPLC.

Immobilized Recombinant Human FGF1 at 5μg/mL (100 μL/well) can bind Recombinant Human FGFR3 with a linear range of 0.5-1.5 μg/mL.

Recombinant Human FGF1 promotes the proliferation of BALB/c 3T3 mouse embryonic fibroblasts cells. The ED₅₀ for this effect is typically 22-140 pg/mL.

All(4)|

货号: RP01242

详细信息

种属

Human

表达宿主

E. coli

Calculated MW

16.67 kDa

Observed MW

20 kDa

标签

C-His

纯度

> 95% by SDS-PAGE.

内毒素

< 1.0 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of 50mM MOPS,100mM Na2SO4,10mM EDTA, pH7.9Contact us for customized product form or formulation.

描述

Recombinant Human FGF-1/aFGF Protein is produced by E. coli expression system. The target protein is expressed with sequence (Phe16-Asp155) of human FGF acidic/FGF1 (Accession #NP_000791.1) fused with a 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human FGF1 at 5 μg/mL (100 μL/well) can bind Recombinant Human FGFR3 with a linear range of 0.5-1.5 μg/mL.
2.Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED₅₀ for this effect is typically 22-140 pg/mL.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

基因ID

2246

Swiss Prot

P05230-1

别名

AFGF; ECGF; ECGF-beta; ECGFA; ECGFB; FGF-1; FGF-alpha; FGFA; GLIO703; HBGF-1; HBGF1;FGF1;ECGF;ECGF-beta;ECGFA;ECGFB;FGF-1;FGF-alpha;FGFA;GLIO703;HBGF-1;HBGF1