Recombinant Rat ALK-1/ACVRL1 Protein (RP01925)

说明书收藏

Recombinant Rat ALK-1/ACVRL1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 45-60 kDa.

All(1)|

货号: RP01925

详细信息

种属

Rat

表达宿主

HEK293 cells

Calculated MW

36.97 kDa

Observed MW

45-60 kDa

标签

C-hFc

纯度

> 90% by SDS-PAGE.

内毒素

< 0.01EU/μg of the protein by LAL method

制剂

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

描述

Recombinant Recombinant Rat ALK-1/ACVRL1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Lys21-Ala118) of Rat ALK-1/ACVRL1 (Accession #NP_071886.1) fused with a hFc tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured by its binding ability in a functional ELISA. Immobilized Rat ALK-1(Catalog #RP01925) at 4 μg/mL (100 μL/well) can bind Human ACVR2B (Catalog #RP00153) with a linear range of 20-989.42 ng/mL.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

ALK-1/ACVRL1,Type I receptor for TGF-beta family ligands BMP9/GDF2 and BMP10 and important regulator of normal blood vessel development. On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. May bind activin as well.

基因ID

25237

Swiss Prot

P80203

别名

Acvrl1; Acvrlk1;Serine/threonine-protein kinase receptor R3; SKR3; 2.7.11.30; TGF-B superfamily receptor type I; TSR-I