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活性验证

Recombinant SARS-COV-2 Spike S1+S2 ECD(S-ECD) Protein (RP01260LQ)

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Recombinant SARS-COV-2 S1+S2 ECD (S-ECD) Protein with His tag was determined by SDS-PAGE with Coomassie Blue, showing bands around 80,110,180 kDa.

Immobilized Recombinant SARS-COV-2 Spike S1+S2 ECD-His at 2μg/mL (100 μL/well) can bind Recombinant Human ACE2 with a linear range of 0.15-9.92 ng/mL.

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货号: RP01260LQ
促销价：   ￥8000
货    期：现货产品
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详细信息

种属
SARS-COV-2
表达宿主
HEK293 cells
Calculated MW
14.11 kDa
Observed MW
80 kDa, 110 kDa, 180 kDa
标签
C-His
纯度
>97% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Supplied as a 0.22 μm filtered solution in PBS, pH 7.4. Contact us for customized product form or formulation.
描述
Recombinant SARS-COV-2 Spike S1+S2 ECD(S-ECD) Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val11-Gln1208) of SARS-COV-2(2019-nCoV) S1+S2 ECD (S-ECD) (Accession #YP_009724390.1) fused with a 6×His tag at the C-terminus.
储存
Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 Spike S1+S2 ECD-His at 2 μg/mL (100 μL/well) can bind Recombinant Human ACE2 with a linear range of 0.15-9.92 ng/mL.

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文献引用 (4)

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Publishing research using RP01260LQ? Please let us know so that we can cite the reference in this datasheet.

背景信息

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates thisinteraction.The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity.

基因ID
43740568
别名
Envelope;SARS-CoV-2 Spike RBD (N501Y);Spike;Spike ECD;Spike RBD;Spike S1;Spike S2;Spike S2 ECD;S1-RBD protein;NCP-CoV RBD Protein;novel coronavirus RBD Protein;2019-nCoV RBD Protein;S glycoprotein Subunit1 RBD Protein
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