Recombinant Human IL-13 Protein (RP01320)

Recombinant Human IL-13 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 15&25-35kDa.

Recombinant Human IL-13 stimulates cell proliferation of the TF-1 Human erythroleukemic cells. The ED₅₀ for this effect is 1-4 ng/mL, corresponding to a specific activity of 0.25 × 10⁶~1.0× 10⁶ units/mg.

All(2)|

货号: RP01320

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

13.18 kDa

Observed MW

15 kDa, 25-35 kDa

标签

C-His

纯度

> 95% by SDS-PAGE.

内毒素

< 0.1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

描述

Recombinant Human IL-13 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly35-Asn146) of Human IL13 (Accession #NP_002179.2) fused with an 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured in a cell proliferation assay using TF-1 Human erythroleukemic cells. The ED₅₀ for this effect is 1-4 ng/mL, corresponding to a specific activity of 0.25 × 10⁶~1.0× 10⁶ units/mg.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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Publishing research using RP01320? Please let us know so that we can cite the reference in this datasheet.

背景信息

Interleukin 13 (IL13) is also known as ALRH,and P600, is a single-chain glycosylated polypeptide, and is a cytokine critical in regulating inflammatory and immune responses. IL13 is secreted by many cell types, but especially by T helper type 2 (Th2) cells. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Rα) and at least one of two known IL-13-specific binding chains.As a cytokine, IL-13 protein is critical in regulating inflammatory, immune responses, and diseases. Also, it inhibits the production of pro-inflammatory cytokines and chemokines, and thus down-regulates macrophage activity. IL-13 protein and antibody are more importantly implicated as a central mediator of immunoregulatory processes in various cell types.

基因ID

3596

Swiss Prot

P35225

别名

IL13;IL-13;P600

研究领域