Recombinant Human MUC-16/CA125 Protein (RP01417)

Recombinant Human MUC-16/CA125 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 45-70kDa.

Immobilized Human CA125 at 5 μg/mL (100 μL/well) can bind Human Mesothelin/MSLN with a linear range of 0.3-17 ng/mL.

Loaded Human Mesothelin/MSLN Protein, C-hFc&His (Catalog: RP01220) on ProA Biosensor, can bind Human CA125 Protein, C-His&Avi (Catalog: RP01417) with an affinity constant of 17.2 nM as determined in BLI assay (Gator).

All(3)|

货号: RP01417

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

2.65 kDa

Observed MW

45-70 kDa

标签

C-His&Avi

纯度

> 95% by SDS-PAGE.

内毒素

< 0.1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

描述

Recombinant Human MUC-16/CA125 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly12660-Met12923) of human CA125/MUC16 (Accession #NP_078966.2) fused with a 6×His，Avi tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Loaded Human Mesothelin/MSLN Protein, C-hFc&His (Catalog: RP01220) on ProA Biosensor, can bind Human CA125 Protein, C-His&Avi (Catalog: RP01417) with an affinity constant of 17.2 nM as determined in BLI assay (Gator).

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

The CA125, also known as the MUC16, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. The CA 125 molecule is almost certainly a glycoprotein with a predominance of O-linkages. It is heterogeneous with regard to both size and charge, most likely due to continuous deglycosylation of side chains during its life-span in bodily fluids. It exists as a very large complex (perhaps as much as 4 million daltons) under natural conditions.

基因ID

94025

Swiss Prot

Q8WXI7

别名

CA125;MUC16;mucin-16

研究领域