Recombinant Human FGF-9 Protein (RP01710)

Recombinant Human FGF-9 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 25-30 kDa.

Recombinant Human FGF-9 stimulates cell proliferation assay using BALB/3T3 mouse fibroblasts. The ED₅₀ for this effect is 2.34-9.34 ng/mL, corresponding to a specific activity of 1.07×10⁵~4.27×10⁵ units/mg.

All(2)|

货号: RP01710

详细信息

种属

Human

表达宿主

E. coli

Calculated MW

23.31 kDa

Observed MW

25-30 kDa

标签

NO-tag

内毒素

<1EU/μg

制剂

Lyophilized from a 0.22 μm filtered solution of 20mM PB, pH 6.0.

描述

Recombinant Human FGF-9 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ala2-Ser208) of human FGF-9 (Accession #NP_002001.1) fused with no additional amino acid.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured in a cell proliferation assay using BALB/3T3 mouse fibroblasts. The ED₅₀ for this effect is 2.34-9.34 ng/mL, corresponding to a specific activity of 1.07×10⁵~4.27×10⁵ units/mg.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

Fibroblast growth factor 9 (FGF9) also known as Glia-activating factor or Heparin-binding growth factor 9, is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells.

基因ID

2254

Swiss Prot

P31371

别名

GAF; FGF-9; SYNS3; HBFG-9; HBGF-9;FGF9