产品和服务 / 产品类型 / 重组蛋白 / 细胞因子 & 细胞因子受体

活性验证

Recombinant Mouse IFN-alpha 2 Protein (RP01725)

文献引用 (2) 评论 (2) 说明书 收藏

Recombinant Mouse IFN-alpha 2 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 20-25 kDa.

Recombinant Mouse IFN-alpha 2 was measured in a cell cytotoxicity assay using TF-1 cells. The ED50 for this effect is 51.80‑207.20 ng/mL, corresponding to a specific activity of 4.83×103~1.93×104 units/mg.

All(2)|
货号: RP01725

促销价：   ￥320
货    期：现货产品
抗体定制服务咨询 |
扫码下单
享受积分

详细信息

种属
Mouse
表达宿主
Pichia
Calculated MW
19.33 kDa
Observed MW
20-25 kDa
标签
NO-tag
内毒素
< 0.1EU/μg
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
描述
Recombinant Mouse IFN-alpha 2 Protein is produced by Pichia expression system. The target protein is expressed with sequence (Cys24-Glu190) of mouse IFN-alpha 2 (Accession #) fused with no additional amino acid.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
Measured in a cell cytotoxicity assay using TF-1 cells. The ED50 for this effect is 51.80‑207.20 ng/mL, corresponding to a specific activity of 4.83×103~1.93×104 units/mg.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
=
÷

客户数据及评论 (2)

折叠内容
CustomerNov 122024
评分
Experiment Type
Cell-based assay
Sample
L929
Description
After 10 ng/mL IFNA2 (Cat.RP01725) was used to stimulate L929 cells for 2h, 4h, 8h and 12h, WB was used to detect the change of ZBP1 protein expression in cells (the same amount of protein samples), and the results showed that IFNA2 could stimulate L929 cells to highly express ZBP1 protein
CustomerNov 122024
评分
Experiment Type
Cell-based assay
Sample
L929
Description
After 10 ng/mL IFNA2 (Cat.RP01725) was used to stimulate L929 cells for 2h, 4h, 8h and 12h, WB was used to detect the change of ZBP1 protein expression in cells (the same amount of protein samples), and the results showed that IFNA2 could stimulate L929 cells to highly express ZBP1 protein

文献引用 (2)

折叠内容

Publishing research using RP01725? Please let us know so that we can cite the reference in this datasheet.

背景信息

IFNA2 (Interferon Alpha 2) is a Protein Coding gene. This gene is a member of the alpha interferon gene cluster on chromosome 9. The encoded protein is a cytokine produced in response to viral infection. Type I Interferons (IFNs) are well-known cytokines that exert antiviral activity, antitumor activity, and immunomodulatory effects. Interferon tau (IFNT), a type I IFN similar to alpha IFNs (IFNA), is the pregnancy recognition signal produced by the ruminant conceptus. Among the IFN-α genes, a total of 28 different sequence variants have been described. The three principal subtypes of IFNα-2 are designated α-2a, α-2b, and α-2c. IFNα-2b is being the predominant allele while IFNα-2a is less predominant and IFNα-2c only a minor allelic variant.

基因ID
15965
Swiss Prot
P01573
别名
IFNA2; IFN-alphaA; IFNA; IFNA2B; INFA2; interferon alpha-2;Interferon alpha 2 (IFN-α2）;IFN-alphaA;IFNA;IFNA2B;INFA2
>