Recombinant Mouse E-Cadherin/CDH1/CD324 Protein (RP01727)

Recombinant Mouse E-Cadherin/Cadherin-1/CDH1 Protein was resolved with SDS PAGE under reducing (R) and non-reducing (NR) conditions, showing single bands at 75 kDa and 150 kDa, respectively.

Recombinant Mouse CDH1 measured by the ability of the immobilized protein to support the adhesion of the MCF-7 human breast cancer cells. The ED₅₀ for this effect is 0.82-3.29 μg/mL.

All(2)|

货号: RP01727

详细信息

种属

Mouse

表达宿主

HEK293 cells

Calculated MW

61.70 kDa

Observed MW

75 kDa

标签

C-6His

内毒素

< 0.1EU/μg

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

描述

Recombinant Mouse E-Cadherin/CDH1/CD324 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp157-Val709) of mouse E-Cadherin/Cadherin-1/CDH1 (Accession #NP_033994.1) fused with and a 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured by the ability of the immobilized protein to support the adhesion of the MCF-7 human breast cancer cells. The ED₅₀ for this effect is 0.82-3.29 μg/mL.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

This protein is a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date.

基因ID

12550

Swiss Prot

P09803

别名

Um; UVO; Ecad; ARC-1; E-cad; L-CAM;CDH1