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活性验证

Recombinant Rat Hepatocyte growth factor/HGF Protein (RP01776)

说明书 收藏

Recombinant Rat Hepatocyte growth factor/HGF Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 80-100 kDa.

Recombinant Rat HGF stimulates cell proliferation of the Mv.1.lu cells. The ED50 for this effect is 1.6-6.2 ng/mL, corresponding to a specific activity of 1.6×105~6.3×105 units/mg.

All(2)|
货号: RP01776

促销价：   ￥780
货    期：现货产品
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详细信息

种属
Rat
表达宿主
HEK293 cells
Calculated MW
80.14 kDa
Observed MW
80-100 kDa
标签
C-His
纯度
> 92% by SDS-PAGE.
内毒素
<0.001EU/μg
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
描述
Recombinant Rat Hepatocyte growth factor/HGF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln 33-Leu728) of rat HGF (Accession #NP_058713.1) fused with and a 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
Measured in a cell proliferation assay using Mv.1.lu cells. The ED50 for this effect is 1.6-6.2 ng/mL, corresponding to a specific activity of 1.6×105~6.3×105 units/mg.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

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请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

Hepatocyte growth factor/scatter factor (HGF/SF) is a paracrine cellular growth, motility and morphogenicfactor. It belongs to the peptidase S1 family and Plasminogen subfamily, contains 4 kringle domains, 1 PANdomain and 1 peptidase S1 domain. HGF regulates cell growth, cell motility, and morphogenesis by activatinga tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. HGF is secreted bymesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability tostimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis,and tissue regeneration.

基因ID
24446
Swiss Prot
P17945
别名
HPTA；Hepatocyte growth factor；HGF

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