Recombinant Human Orexin-A/Hypocretin/HCRT Protein (RP01920)

Recombinant Human Orexin A/Hypocretin-1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 35-40 kDa.

All(1)|

货号: RP01920

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

29.54 kDa

Observed MW

35-40 kDa

标签

N-hFc

纯度

> 90% by SDS-PAGE.

内毒素

< 0.1EU/μg of the protein by LAL method

制剂

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

描述

Recombinant Human Orexin-A/Hypocretin/HCRT Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln34-Leu66) of Human Orexin A/Hypocretin-1 (Accession #NP_001515.1) fused with a hFc tag at the N-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

Orexin A/Hypocretin-1,Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggestedBy Similarit，Orexin-A，Binds to orexin receptors HCRTR1/OX1R and HCRTR2/OX2R with a high affinity .Stimulates food intake.Modulates pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner.

基因ID

3060

Swiss Prot

O43612

别名

HCRT; OX; PPORX; PPOX;Hypocretin neuropeptide precursor; Hypocretin; Hcrt; Orexin precursor; Prepro-orexin; Preprohypocretin; Cleaved into: Orexin-A; Hypocretin-1; Hcrt1; Orexin-B; Hypocretin-2; Hcrt2

研究领域