产品中心 / 产品类型 / 重组蛋白 / 生物标志物 & CD分子

活性验证

Recombinant Human CD3E&CD3D Heterodimer Protein (RP02024)

说明书 联系我们 收藏

Human CD3E&CD3D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Human CD3E&CD3D was greater than 95% as determined by SEC-HPLC.

Immobilized Human CD3E&CD3D, hFc Tag at 1μg/ml (100μl/Well). Dose response curve for Anti-CD3E&CD3D Ab., mFc Tag (OKT3) with the EC50 of 28.8/25.5/27.8ng/ml determined by ELISA.

Immobilized Human CD3E&CD3D, hFc Tag at 1μg/ml (100μl/Well). Dose response curve for Anti-CD3E&CD3D Ab., mFc Tag (OKT3) with the EC50 of 23.8/22.6/23.5/22.8ng/ml determined by ELISA.

Immobilized Human CD3E&CD3D, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-CD3E&CD3D Ab.1, hFc Tag with the EC50 of 31.68ng/ml determined by ELISA.

Immobilized Human CD3E&CD3D, hFc Tag at 0.1μg/ml (100ul/Well). Dose response curve for Anti-CD3E&CD3D Ab.2, mFc Tag with the EC50 of 27.0ng/ml determined by ELISA.

All(6)|
货号: RP02024
促销价：   ￥3750
货    期：在线咨询货期
抗体定制服务咨询

详细信息

种属
Human
表达宿主
HEK293 cells
描述
Recombinant Human BAFFR Protein is produced by mammalian expression system. The target protein is expressed with sequence (Asp23-Asp126 (CD3E) & Phe22-Ala105 (CD3D)) of human CD3 epsilon & CD3 delta Heterodimer (Accession #P07766 & P04234) fused with an Fc tag at the C-terminus.
标签
C-hFc
纯度
> 95% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
生物活性
Immobilized Human CD3 epsilon & CD3 delta Heterodimer Protein at 0.5 μg/mL (100 μL/well), dose response curve for Anti-hCD3e mAb with the EC50 of 21.69 ng/mL determined by ELISA
制剂
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
储存
Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
=
÷

背景信息

T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.

基因ID
915
916
Swiss Prot
P07766 & P04234
别名
CD3-DELTA; IMD19; T3D;IMD18; T3E; TCRE
研究领域
>