Recombinant Human GFR alpha-like/GFRAL Protein (RP02211)

说明书收藏

Human GFRAL on Tris-Bis PAGE under reduced condition.The purity is greater than 95%.

The purity of Human GFRAL is greater than 95% as determined by SEC-HPLC.

Immobilized Human GFRAL, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human GDF15, hFc Tag with the EC₅₀ of 9.4ng/ml determined by ELISA.

All(3)|

货号: RP02211

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

40.7 kDa

Observed MW

48-62 kDa

标签

C-His&Avi

纯度

> 95% as determined by Tris-Bis PAGE.> 95% as determined by HPLC.

内毒素

< 1EU per μg by the LAL method.

制剂

Lyophilized from 0.22um filtered solution in 20mM PB, 300mM NaCl (pH 6.5). Normally 5 % trehalose is added as protectant before lyophilization.

描述

Recombinant Human GFR alpha-like/GFRAL Protein is produced by mammalian expression system. The target protein is expressed with sequence (Ser19-Glu351) of Human GFR alpha-like/GFRAL (Accession #) fused with a His and Avi tag at the C-terminal. .

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses

基因ID

389400

Swiss Prot

Q6UXV0

别名

GFR alpha-like; GFRAL; GRAL; C6orf144