产品中心 / 产品类型 / 重组蛋白 / 免疫检查点

Recombinant Human PD-1/PDCD1/CD279 Protein (RP02237)

说明书 联系我们 收藏

Recombinant Human PD-1/PDCD1/CD279 Protein was determined by SDS-PAGE with Coomassie Blue, showing bands at 43.5 kDa

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human PD1, mFc-His tagged protein can bind Anti-PD-1 Neutralizing antibody in a linear range of 0.24-6.49 ng/ml.

All(2)|
货号: RP02237
促销价：   ￥660
货    期：在线咨询货期
抗体定制服务咨询

文献引用 (0)

详细信息

种属
Human
表达宿主
HEK293 cells
描述
Recombinant Human PD-1/PDCD1/CD279 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (PD-1(Phe24-Val170)&mFc(Pro99-Lys330)) of human PDCD1 (Accession #) fused with a Fc and His Tag at the C-terminal. .
标签
C-mFc&His
纯度
> 95% by SDS-PAGE.
生物活性
ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human PD1, mFc-His tagged protein can bind Anti-PD-1 Neutralizing antibody in a linear range of 0.24-6.49 ng/mL.
制剂
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 32 % trehalose is added as protectants before lyophilization.
储存
Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Reconstitute with deionized water收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
=
÷

背景信息

This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases.

基因ID
5133
Swiss Prot
Q15116
别名
PDCD1; CD279; PD-1; PD1; SLEB2; hPD-1; hPD-l; hSLE1; programmed cell death 1;PD-1;CD279;PD1;SLEB2;hPD-1;hPD-l;hSLE1;PD-1/CD279
研究领域
>