Recombinant Human Fas ligand/APTL/CD95 ligand/CD178 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 25-35 kDa.
Recombinant Human Fas ligand/APTL/CD95 ligand/CD178 induce apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is 2.075-8.3 ng/mL in the presence of 10 µg/mL of a cross-linking antibody Rabbit Anti-poly Histidine Monoclonal Antibody (Catalog # AE086), corresponding to a specific activity of 1.20×105~4.82×105 units/mg.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
描述
Recombinant Human TNFSF6/FAS ligand/CD178 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Pro134-Leu281) of Human Fas Ligand(monomer) fused with a His tag at the N-terminal.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月; 复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
生物活性
Measured by its ability to induce apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is 2.075-8.3 ng/mL in the presence of 10 µg/mL of a cross-linking antibody Rabbit Anti-poly Histidine Monoclonal Antibody (Catalog # AE086), corresponding to a specific activity of 1.20×105~4.82×105 units/mg.
复溶
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。