Biotinylated Recombinant Human P-Selectin/SELP/CD62P Protein (RP02487B)

说明书联系我们收藏

Biotinylated Human P-Selectin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human P-Selectin is greater than 95% as determined by SEC-HPLC.

Immobilized Anti-P-selectin Antibody, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human P-Selectin, His Tag with the EC₅₀ of 0.11μg/ml determined by ELISA.

All(3)|

货号: RP02487B

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

82.9 kDa

Observed MW

115-140 kDa

标签

C-His&Avi

纯度

> 95% by Tris-Bis PAGE and HPLC

内毒素

< 1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization. Contact us for customized product form or formulation.

描述

Biotinylated Recombinant Human P-Selectin/SELP/CD62P Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Trp42-Ala771) of Human P-Selectin/SELP/CD62P fused with His tag and Avi tag at the C-terminus.

储存

Store at -20℃. Store the lyophilized protein at -20℃ to -80℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

Human P-Selectin is a cell surface glycoprotein expressed by activated platelets and endothelial cells. A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation.

基因ID

6403

Swiss Prot

P16109

别名

CD62; CD62P; GMP140; GRMP; LECAM3; PADGEM; PSEL;CD62P/P-selectin;SELP;CD62P;GMP140;GRMP;LECAM3;PADGEM;PSEL

研究领域