Recombinant Human FGFR-2 beta (IIIb)/KGFR/CD332 (253-378) Protein (RP02529)

说明书收藏

Human FGFR2 beta (IIIb) Domain on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Human FGFR2 beta (IIIb) Domain is greater than 95% as determined by SEC-HPLC.

Immobilized Human FGFR2 beta (IIIb) Domain, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC₅₀ 14.3ng/ml determined by ELISA.

All(3)|

货号: RP02529

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

40.5 kDa

Observed MW

60-68 kDa

标签

C-hFc

纯度

> 95% as determined by Tris-Bis PAGE；> 95% as determined by HPLC

内毒素

Less than 1EU per μg by the LAL method.

描述

Recombinant Human FGFR-2 beta (IIIb)/KGFR/CD332 (253-378) Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Pro253-Glu378) of human FGFR-2 beta (IIIb)/KGFR/CD332 (Accession #P21802-3) fused with hFc tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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背景信息

Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha isoform, or only IgII and IgIII, referred to as the beta isoform.

基因ID

2263

Swiss Prot

P21802-3

别名

Fibroblast growth factor receptor 2；FGFR-2；KGFR；K-sam；Keratinocyte growth factor receptor；CD332；BEK；KSAM

研究领域