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Recombinant Human Plasma Kallikrein/KLKB1 Protein (RP02872)

Recombinant Human Plasma Kallikrein/KLKB1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 72-80kDa.

The purity of Human Plasma Kallikrein/KLKB1 Protein (Cat.RP02872LQ) was greater than 95% as determined by SEC-HPLC.

Immobilized Human KLKB1, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-KLKB1 Antibody, hFc Tag with the EC50 of 36.7ng/ml determined by ELISA.

All(3)|
货号: RP02872
促销价:   ¥3000
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详细信息

种属
Human
表达宿主
HEK293 cells
Calculated MW
70.3 kDa
Observed MW
72-80 kDa
标签
C-His
纯度
> 95% as determined by SEC-HPLC.
内毒素
<1EU/μg
制剂
Lyophilized from a 0.22 μm filtered solution of 20mM NaAc,150mM NaCl,pH5.0.
描述
Recombinant Human Plasma Kallikrein/KLKB1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly20-Ala638) of human Plasma Kallikrein/KLKB1 (Accession #NP_000883.2) fused with 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in 20mM NaAc,150mM NaCl,pH5.0.. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度,快速计算您需要添加溶液的体积吧!
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背景信息

Plasma kallikrein, also known as Fletcher factor or kallikrein B1 (KLKB1), is a serine endopeptidase, like its homologs tissue kallikrein and kallikrein-related peptidases (KLKs). Its physiological role is to catalyze the release of kinins and other vasoactive peptides.

基因ID
Swiss Prot
别名
KLKB1;KLK3;PKK;PKKD;PPK
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