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Recombinant Human Insulin Protein (RP02930)

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Recombinant Human Insulin Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 7 kDa.

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货号: RP02930
促销价：   ￥3800
货    期：在线咨询货期
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文献引用 (1)

折叠内容

Publishing research using RP02930? Please let us know so that we can cite the reference in this datasheet.

详细信息

种属
Human
表达宿主
Yeast
描述
Recombinant Human Insulin Protein is produced by Yeast expression system. The target protein is expressed with sequence ((Phe25-Lys53)AAK(Gly90-Asn110)) of human Insulin (Accession #NP_000198.1) fused with no additional amino acid.
标签
No tag
纯度
> 95% by SDS-PAGE.
内毒素
Please contact us for more information
生物活性
Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells.The ED50 for this effect is typically 0.2-1 μg/mL.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
储存
Store the lyophilized protein at -20°C to -80°C for 12 months.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

INS (Insulin) is a Protein Coding gene. This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. The binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Diseases associated with INS include Hyperproinsulinemia and Maturity-Onset Diabetes Of The Young, Type 10. A multitude of mutant alleles with phenotypic effects has been identified, including insulin-dependent diabetes mellitus, permanent neonatal diabetes mellitus, maturity-onset diabetes of the young type 10, and hyperproinsulinemia.

基因ID
3630
Swiss Prot
P01308
别名
IDDM;IDDM1;IDDM2;ILPR;IRDN;MODY10;INS;Insulin;insulin
研究领域
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