Recombinant Human HER1/ERBB1/EGFR (668-1210) Protein (RP02943LQ)

Recombinant Human EGFR (aa668-1210) Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 90-100 kDa.

All(1)|

货号: RP02943LQ

详细信息

种属

Human

表达宿主

Baculovirus-Insect Cells

Calculated MW

89.1 kDa

Observed MW

89 kDa

标签

N-His＆GST

纯度

> 75% by SDS-PAGE.

内毒素

< 1 EU/μg of the protein by LAL method.

制剂

Supplied as a 0.22 μm filtered solution in 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4.

描述

Recombinant Human ERBB1/HER1/EGFR (668-1210) Protein is produced by Baculovirus-Insect Cells expression system. The target protein is expressed with sequence (Met668-Ala1210) of Human ERBB1/HER1/EGFR (668-1210) (Accession #NP_005219.2.) fused with an 6×His, GST tag at the N-terminus.

储存

Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured by its binding ability in a functional ELISA. Immobilized recombinant human EGF at 0.5 μg/mL (100 μL/well) can bind Rrecombinant human EGFR with a linear range of 0.3-2.5 μg/mL.

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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背景信息

The EGFR (epidermal growth factor receptor) subfamily of receptor tyrosine kinases comprises four members: EGFR (also known as HER-1, ErbB1, or ErbB), ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc.The human EGFR cDNA encodes a 1210 amino acid (aa) precursor with a 24?aa signal peptide, a 621 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 542 aa cytoplasmic domain. EGFR signaling has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently, which is overexpressed in a wide variety of tumors and is the target of several anti-cancer drugs.

基因ID

1956

Swiss Prot

P00533

别名

EGFR; ERBB; ERBB1; HER1; NISBD2; PIG61; mENA; epidermal growth factor receptor;ERBB;ERBB1;HER1;NISBD2;PIG61;mENA

研究领域