Recombinant Human KRAS-G13D Protein was determined by SDS-PAGE with Coomassie Blue, showing bands at 23.6 kDa.
KRAS-G13D activity test using HTRF method. The KRAS-G13D activity was assayed with HTRF technology. The reaction was performed by incubating the KRAS-G13D protein, GTP, cRAF and beads at 25℃ for 60 min, then reading Ratio 665/620nm signal with BMG.
Recombinant human KRAS-G13D protein with N-terminal 8x his tag+ TEV cleavage site and C-terminal Avi tag was purified by Ni-NTA affinity and followed by SEC chromatography. The KRAS-G13D protein showed high activity in HTRF assay.
标签
N-8His
纯度
> 95% by SDS-PAGE
生物活性
KRAS-G13D activity test using HTRF method.
制剂
Supplied as a 0.22 μm filtered solution in 20mM Tris, 150mM NaCl, 1mM DTT, 10% glycerol, pH7.4
储存
This product is stable at ≤ -70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月; 复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。