Recombinant Human TRIM21 Protein is produced by Baculovirus-Insect cells expression system. The target protein is expressed with sequence (Met1-Tyr475) of human TRIM21 (Accession #NP_003132.2) fused with polyhistidine tag at the N-terminus.
标签
N-His
纯度
> 80% by SDS-PAGE.
内毒素
< 1.0 EU/μg of the protein by LAL method.
制剂
Supplied as sterile 20 mM Tris, 500 mM NaCl, 20% Glycerol, 0.2 M Arg, 1 mM TCEP, pH 8.0.
储存
Store at ≤-70°C, stable for 12 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月; 复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
复溶
Please contact us for more information.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。