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Recombinant Human IL-27/IL-27A&IL-27B Protein (RP03226)

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Recombinant Human IL-27/IL-27A&IL-27B Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 54-64 kDa.

Immobilized Human IL-27 RA, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 55.9ng/ml determined by ELISA (QC Test).

Human IL-27 RA, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-27, His Tag with an affinity constant of 3.58 nM as determined in SPR assay (Biacore T200).

All(3)|
货号: RP03226
促销价：   ￥4800
货    期：在线咨询货期
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详细信息

种属
Human
表达宿主
CHO cells
描述
Recombinant Human IL-27 Protein is produced by Human cells expression system. The target protein is expressed with sequence (Arg21-Lys229(IL-27B)&Phe29-Pro243(IL-27A)) of Human IL-27 (Accession #Q8NEV9＆Q14213) fused with a 6×His tag at the C-terminus.
标签
C-His
纯度
> 95% by SDS-PAGE.
内毒素
< 1 EU/μg of the protein by LAL method.
生物活性
1.Immobilized Human IL-27 RA, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 55.9ng/ml determined by ELISA (QC Test)
2.Human IL-27 RA, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-27, His Tag with an affinity constant of 3.58 nM as determined in SPR assay (Biacore T200).
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, 200mM L-arginine (pH 7.4). Contact us for customized product form or formulation.
储存
Store the lyophilized protein at -20°C to -80°C for 12 months.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

Interleukin (IL)-27, a member of the IL-6/IL-12 family, has an important role in modulating inflammation in partnership with innate and adaptive immune cells.

基因ID
246778＆10148
Swiss Prot
Q8NEV9＆Q14213
别名
Interleukin-27; p28; IL30; IL-27; IL-27A; IL27; p28
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