Recombinant Human Phospholipase A2/PLA2G1B Protein (RP03277)

Recombinant Human Phospholipase A2/PLA2G1B Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 15-20 kDa.

All(1)|

货号: RP03277

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

17.20 kDa

Observed MW

15-20 kDa

标签

C-His

纯度

> 92% by SDS-PAGE.

内毒素

< 1.0 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of 10 mM Tris, 5 mM CaCl₂, pH 8.0. Contact us for customized product form or formulation.

描述

Recombinant Human Phospholipase A2/PLA2G1B Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Ser148) of human Phospholipase A2/PLA2G1B (Accession #NP_000919.1) fused with His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured by its ability to hydrolyze 1-Hexadecanoyl-2-(1-pyrenedecanoyl)-sn-glycero-3-phosphomethanol. The specific activity is >2500 pmol/min/μg.

复溶

Please contact us for reconstitution instructions.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

Phospholipase A2 (PLA2) plays crucial roles in diverse cellular responses, including phospholipid digestion and metabolism, host defense and signal transduction. PLA2 provides precursors for generation of eicosanoids, such as prostaglandins (PGs) and leukotrienes (LTs), when the cleaved fatty acid is arachidonic acid, platelet-activating factor (PAF) when the sn-1 position of the phosphatidylcholine contains an alkyl ether linkage and some bioactive lysophospholipids, such as lysophosphatidic acid (lysoPA).

基因ID

5319

Swiss Prot

P04054

别名

Phospholipase A2; PLA2G1B; PLA2; PLA2A; PPLA2

研究领域