产品和服务 / 产品类型 / 细胞系&裂解液 / KO细胞系

活性验证

Recombinant Human EGF Protein (RP03287)

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Human gastric cancer organoid were cultured with EGF(Cat. RP03287), NOG(Cat. RP01237), FGF10(Cat. RP01140), RSPO1(Cat. RP00071), Wnt-3a Surrogate(Cat. RP01618SLQ).

Human colorectal cancer organoid were cultured with EGF(Cat. RP03287), NOG(Cat. RP01237)， FGF10(Cat. RP01140), RSPO1(Cat. RP00071), HGF(Cat. RP01602).

Human liver organoids were cultured with EGF(Cat. RP03287), HGF(Cat. RP01602), FGF2(Cat. RP01042), FGF10(Cat. RP01140), NOG(Cat. RP01237), RSPO1(Cat. RP00071), WNT-3a(Cat. RP01618SLQ).

Measured in a cell proliferation assay using Balb3T3 mouse fibroblast cells. The ED50 for this effect is 0.04-0.17 ng/mL, corresponding to a specific activity of 5.88×106~2.50×107 units/mg.

Human stomach organoids were cultured with EGF(Cat. RP03287), FGF10(Cat. RP01140), NOG(Cat. RP01237), RSPO1(Cat. RP00071), WNT-3a(Cat. RP01618SLQ).

Mouse lung organoids were cultured in Matrigel (Cat. RPM0003) with medium supplemented with EGF (Cat. RP03287), Noggin (Cat. RP01237), RSPO1 (Cat. RP00071), Wnt-3a (Cat. RP01618SLQ), and FGF-10 (Cat. RP01140).

Recombinant Human EGF Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human kidney organoids were cultured with EGF(Cat. RP03287), FGF2(Cat. RP01042), FGF7(Cat. RP01717), FGF9(Cat. RP01710), FGF10(Cat. RP01140), IGF-(Cat. RP00996), NOG(Cat. RP01237), RSPO1(Cat. RP00071), WNT-3a(Cat. RP01618SLQ). And further, DKK-1(RP01343) was used to induce the establishment of cell polarity.

Recombinant Human EGF Protein is greater than 95% as determined by SEC-HPLC.

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货号: RP03287
价 格：  380.00
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详细信息

靶点
EGF
细胞系
6.35 kDa
突变描述
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.
敲除验证
5-15 kDa
产品组成
Asn971-Arg1023
物种
E. coli
保存条件
Store at -20℃
运输条件
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

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折叠内容
中国科学院动物研究所Nov 282025
评分
Experiment Type
Cell-based assay
Sample
hPSC
Description
The differentiation medium containing RSPO1 (500 ng/mL), Noggin (100 ng/mL), and EGF (100 ng/mL) was used to induce the differentiation of mid-hindgut cells into small intestinal organoids. After 7 days of induction, the expression of markers CDX2, MUC2, CgA, and E-Cad was assessed by immunofluorescence.
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背景信息

As a low-molecular-weight polypeptide, EGF was first purified from the mouse submandibular gland, but since then it was found in many human tissues including submandibular gland, parotid gland. It can also be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is a growth factor that stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. It results in cellular proliferation, differentiation, and survival. Salivary EGF, which seems also regulated by dietary inorganic iodine, also plays an important physiological role in the maintenance of oro-esophageal and gastric tissue integrity. EGF acts by binding with high affinity to epidermal growth factor receptor on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor.

基因ID
1950
Swiss Prot
P01133
别名
EGF;Pro-epidermal growth factor; EGF; Cleaved into: Epidermal growth factor; Urogastrone
研究领域