Stable transfected mouse cell line overexpressing human Nectin-4
Description
Flow cytometry analysis of Stable transfected mouse cell line overexpressing human Nectin-4, using ABflo® 647 Rabbit IgG isotype control (A22070) and ABflo® 647 Rabbit anti-Human Nectin-4 mAb (A25193) at 1:40 dilution. clearly classified positive and negative cells.
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen.An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.ABflo? 647 Excitation: 651 nm; Emission: 671 nm; Laser: Red Laser.
