Western blot analysis of lysates from wild type (WT) and 293F cells transfected with NFAT-HA-Tag(C terminal) using HA-Tag Rabbit mAb (AE105) at 1:5000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.
Immunofluorescence analysis of 293T-SCRNI-HA-GFP(C) and 293T cells using HA-Tag Rabbit mAb (AE105) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of 293T-SCRNI-HA-GFP(N) and 293T cells using HA-Tag Rabbit mAb (AE105) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300 μg extracts of 293T-SCRN1-HA-GFP-N cells using 3 μg HA-Tag Rabbit mAb (AE105). Western blot was performed from the immunoprecipitate using HA-Tag Rabbit mAb (AE105) at a dilition of 1:6000.
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