产品信息
产品描述
ABclonal Scale ssDNA-seq Lib Prep Kit for Illumina V2 是一款通用型、快速、高效的 Illumina 测序文库构建的试剂盒，以片段化的单链 DNA 起始建库，适用于各种应用样本类型，包括 gDNA、FFPE DNA、Cell-free DNA、ChIP DNA、古生菌、病毒 DNA及 RNA-seq的单链 cDNA 等。Scale ssDNA-seq Lib Prep Kit V2可以满足 10 pg~200 ng 样本 DNA 的高效文库制备。ABclonal Scale ssDNA-seq Lib Prep Kit for Illumina V2相对于V1版本改良了连接效率，增加了文库产量，提高了低起始量样本的建库成功率。
产品特点
- 非 T-A 依赖的 DNA 连接技术建库试剂盒；
- 实现了 DNA 连接效率的最大化，不再受限制于 DNA 末端完整性、DNA 起始量、接头浓度等条件；
- 兼容 10 pg~200 ng DNA 起始量；
- 适用于双链或者单链等各种类型 DNA 样本。
产品保存
-15~25℃保存
产品组分
| 试剂盒模块 | 试剂管名称与颜色 | 8 次 | 24 次 | 96 次 |
| 预处理 | Low-EDTA TE | 1 mL X 2 | 5 mL | 25 mL |
| T7 Tailing & Ligation | T7 Buffer | 32 μL | 96 μL | 384 μL |
| T7 Adapter | 20 μL | 60 μL | 240 μL |
| T7 Enzyme Mix II | 24 μL | 72 μL | 288 μL |
| Second Strand Synthesis | 2X Synthesis Mix | 344 μL | 1.032 mL | 4.128 mL |
| Synthesis Reagent | 24 μL | 72 μL | 288 μL |
| T5 Adapter Ligation | T5 Buffer II | 64 μL | 192 μL | 768 μL |
| T5 Adapter II | 40 μL | 120 μL | 480 μL |
| Ligase Mix | 24 μL | 72 μL | 288 μL |
| Amplification | 2X PCR Master Mix | 200 μL | 600 μL | 1.2 mL X 2 |
FAQs
Publishing research using RK20228? Please let us know so that we can cite the reference in this datasheet.
Primase promotes the competition between transcription and replication on the same template strand resulting in DNA damage - PMC
Journal
Nature communications
Species
Arabidopsis thaliana
Replication protein RPA2A regulates floral transition by cooperating with PRC2 in Arabidopsis
Journal
The New phytologist
Species
Arabidopsis thaliana
Plant‐specific histone deacetylases associate with ARGONAUTE4 to promote heterochromatin stabilization and plant heat tolerance
Journal
The New phytologist
Species
Nicotiana benthamiana
说明书
说明书下载
