产品信息
产品描述
ABclonal First Strand Synthesis Module (Stranded) (RK20342)适用于RNA-seq文库构建中RNA片段化和first strand cDNA合成。First strand cDNA产物无需纯化可直接进行后续的second strand cDNA的合成。该模块中的RT Strand Specificity Reagent含有Actinomycin D以提高链特异性,可搭配ABclonal Second Strand Synthesis Module (Stranded) (RK20343)使用制作链特异性RNA文库。另外,Frag/Elute Buffer是通过Mg2+高温条件对RNA进行片段化,因而用以打断的RNA样品中不能含有Mg2+等金属离子或EDTA等金属离子螯合剂等,否则会影响RNA的片段化大小。
产品保存
-20℃保存
产品组分
试剂组分 | 24次(RK20342M) | 96次(RK20342L) | 500次(RK20342XL) |
2×Frag/Elute Buffer | 132μL | 528 μL | 1.25 mL ×2 |
RT Strand Specificity Reagent | 192μL | 768 μL | 4 mL |
First Strand Synthesis Enzyme Mix | 48μL | 192 μL | 1 mL |
FAQs
Publishing research using RK20342? Please let us know so that we can cite the reference in this datasheet.
Arsenic exposure induced renal fibrosis via regulation of mitochondrial dynamics and the NLRP3TGF1/SMAD signaling pathway
Journal
Environmental toxicology
说明书
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