产品信息
产品简介
Note: Paraffin tissue sections, frozen tissue sections, cell slides (Frozen sections and cell slides need to be used with RM02984P antibody elution buffer dedicated for multiplex fluorescence staining).
- The primary principle of this product is based on Tyramide Signal Amplification (TSA) technology. The TSA principle utilizes the peroxidase reaction of tyramide (i.e., HRP catalyzes H₂O₂ to activate fluorescently labeled tyramide salts, forming covalent binding sites), generating a substantial enzymatic reaction. The reaction product binds to surrounding protein residues (including tryptophan, histidine, and tyrosine residues), resulting in the deposition of a large amount of fluorophore at the antigen-antibody binding site to achieve signal amplification. Simultaneously, through antibody stripping, multiple fluorescence staining can be achieved using different fluorescent tyramides.
产品特点
- 1.High Sensitivity:Based on Tyramide Signal Amplification (TSA) technology, it offers ultra-high sensitivity and provides more accurate detection results.
- 2.Cost-Effective:Unlike traditional immunofluorescence, TSA allows the use of primary antibodies from the same or different species. Secondary antibodies are HRP-conjugated, and multiple dyes can be used for multiplex protein labeling.
- 3.High Stability:The fluorescence is resistant to quenching, ensuring extremely high product stability.
产品应用
Suitable for multiplex immunofluorescence experiments on paraffin sections,frozen sections,and cell slides.
产品组成
| Product Code | Product Name | Size（10 T） | Size（50 T） | Storage Temperature |
| RK50025P-1 | TYR-520 plus fluorochrome（200×） | 5 μL（Concentrated type） | 25 μL（Concentrated type） | 2-8℃ , Protect from Light |
| RK50025P-2 | TYR-570 plus fluorochrome（200×） | 5 μL（Concentrated type） | 25 μL（Concentrated type） | 2-8℃, Protect from Light |
| RK50025P-3 | TSA buffer | 1.6 mL | 8 mL | 2-8℃ |
| RK50025P-4 | Polymer-HRP goat anti-rabbit secondary antibody（Ready-to-use） | 1.6 mL | 8 mL | 2-8℃ |
| RK50025P-5 | DAPI staining solution（Ready-to-use） | 1 mL | 5 mL | 2-8℃, Protect from Light |
| RK50025P-6 | Antibody diluent | 2 mL | 10 mL | 2-8℃ |
| RK50025P-7 | Peroxidase inhibitor solution | 2 mL | 10 mL | 2-8℃, Protect from Light |
| RK50025P-8 | Antifade mounting medium | 1 mL | 5 mL | 2-8℃ |
注意事项
- 1.Please read the instruction manual carefully before starting the experiment.
- 2.This product is for Research Use Only. Not for use in diagnostic procedures or any other clinical applications.
- 3.For your safety and health, please wear a lab coat and disposable gloves when handling this product.
保存条件和运输
Store at 2-8℃ for 12 months.
说明书
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