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Rabbit anti-Human VEGF165 mAb (RM17780)

This standard curve is only for demonstration purposes. A standard curve should be generated for eachassay.

Fromthe comparison of the standard curve, it is found that ABclonal has highsensitivity.

Placed at 37°C for 3 days, the stability of the standard curve all conformto CV <10%.

All(3)|
货号: RM17780
促销价:   ¥2100
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详细信息

产品名称
Rabbit anti-Human VEGF165 mAb
货号
RM17780
免疫原
Recombinant Human VEGF165 Protein
纯化方式
Affinity purification
同种型
Rabbit IgG
存储溶液
Supplied as a 0.2umfiltered solution in PBS with0.05%ProClin 300 ,Ph7.4 containing Human VEGF165 Antibody.
CrossReactivity
No significant cross-reactivity or interference between VEGF165 and analogues was observed.
存储条件
Store at -20℃. Avoid freeze / thaw cycles. Preservative:0.05%ProClin 300.Avoid repeated freeze-thaw cycles.

* This package insert must be read in its entirety before using this product.

应用

Human VEGF165 Sandwich ELISA Immunoassay

 Recommended
Concentration
Sample
ELISA Capture0.5-2ug/mLRabbit anti-Human VEGF165 (CAP)(Cat. No.RM17780)
ELISA Detection0.005-0.02ug/mLRabbit anti-Human VEGF165 (DET)(Cat. No.RM17781)
Standard31.2-2000pg/mLRecombinant Human VEGF165 Protein(Cat. No.RP01150)

背景信息

This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. The levels of VEGF are increased during infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), thus promoting inflammation by facilitating recruitment of inflammatory cells, and by increasing the level of angiopoietin II (Ang II), one of two products of the SARS-CoV-2 binding target, angiotensin-converting enzyme 2 (ACE2). In turn, Ang II facilitates the elevation of VEGF, thus forming a vicious cycle in the release of inflammatory cytokines.

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